Author Correction: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis | Nature

## Summary
Xavier da Silva, Zhiyi Chen, Sena Atici & José Pedro Friedmann Angeli Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, Heidelberg, Germany Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany Palina Nepachalovich & Maria Fedorova Institute of Genetics, CECAD, University of Cologne, Cologne, Germany Lohans Pedrera & Ana Garcia-Saez Department of Chemistry & Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada Omkar Zilka, Emily L. Covey Research Unit Analytical BioGeoChemistry, Helmholtz Center München (HMGU), Neuherberg, Germany Bernhard Michalke German Cancer Consortium (DKTK), Heidelberg, Germany Andreas Trumpp Authors Florencio Porto Freitas View author publications Search author on: PubMed Google Scholar Hamed Alborzinia View author publications Search author on: PubMed Google Scholar Ancély Ferreira dos Santos View author publications Search author on: PubMed Google Scholar Palina Nepachalovich View author publications Search author on: PubMed Google Scholar Lohans Pedrera View author publications Search author on: PubMed Google Scholar Omkar Zilka View author publications Search author on: PubMed Google Scholar Alex Inague View author publications Search author on: PubMed Google Scholar Corinna Klein View author publications Search author on: PubMed Google Scholar Nesrine Aroua View author publications Search author on: PubMed Google Scholar Kamini Kaushal View author publications Search author on: PubMed Google Scholar Bettina Kast View author publications Search author on: PubMed Google Scholar Svenja M. Lorenz View author publications Search author on: PubMed Google Scholar Viktoria Kunz View author publications Search author on: PubMed Google Scholar Helene Nehring View author publications Search author on: PubMed Google Scholar Thamara N. Genaro-Mattos View author publications Search author on: PubMed Google Scholar Karoly Mirnics View author publications Search author on: PubMed Google Scholar Lokender Kumar View author publications Search author on: PubMed Google Scholar Judith Klein-Seetharaman View author publications Search author on: PubMed Google Scholar Svenja Meierjohann View author publications Search author on: PubMed Google Scholar Isabel Weigand View author publications Search author on: PubMed Google Scholar Matthias Kroiss View author publications Search author on: PubMed Google Scholar Georg W.

## Article Content
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Subjects
Cancer metabolism
Cell death
The
Original Article
was published on 31 January 2024
Correction to:
Nature
https://doi.org/10.1038/s41586-023-06878-9
Published online 31 January 2024
In the originally published version of this article, the blank values in Extended Data Fig. 2a,b correspond to background fluorescence measurements of a stable commercial resazurin solution (AquaBluer Cell Viability Assay Solution, Multi-Target Pharmaceuticals, cat. no. 6015), diluted in complete culture medium. The values reported in the dataset are rounded versions of the direct fluorescence readouts obtained from a SpectraMax plate reader during each experiment, rounded to the nearest 50 fluorescence units. The subsequent experiments with a higher background value were acquired using a self-made resazurin solution, as indicated in the materials and methods, and recorded on the SPARK-Tecan plate reader. The blanks were defined as the average of three wells without rounding.
Following identification of the issue, we reanalysed the data to evaluate the potential impact of the blank values on our results, employing two distinct methodologies. First, we recalculated all values using a blank set to zero (see Figs. 1 and 2 in
Supplementary Information
), corresponding to 0% viability. Secondly, we normalised the data more stringently by defining the lowest measured condition — wild-type cells treated with 1 µM RSL3 or 10 µM FIN56 — as zero percent viability (see Figs. 3 and 4 in
Supplementary Information
). In both cases, the resulting plots fundamentally showed the same effects. These corrections do not affect the experiments and the study conclusions.
Supplementary information
is available in the online version of this amendment.
Author information
Author notes
These authors contributed equally: Florencio Porto Freitas, Hamed Alborzinia, Ancély Ferreira dos Santos
Authors and Affiliations
Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg, Würzburg, Germany
Florencio Porto Freitas, Ancély Ferreira dos Santos, Alex Inague, Helene Nehring, Thamara N. Xavier da Silva, Zhiyi Chen, Sena Atici & José Pedro Friedmann Angeli
Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany
Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp
Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, Heidelberg, Germany
Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp
Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany
Palina Nepachalovich & Maria Fedorova
Institute of Genetics, CECAD, University of Cologne, Cologne, Germany
Lohans Pedrera & Ana Garcia-Saez
Department of Chemistry & Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada
Omkar Zilka, Emily L. Schaefer, Ifedapo Ekpo & Derek A. Pratt
Instituto de Química, Universidade de Sao Paulo, Sao Paulo, Brazil
Alex Inague & Sayuri Miyamoto
Institute of Metabolism and Cell Death, Helmholtz Zentrum München, Neuherberg, Germany
Svenja M. Lorenz, Sebastian G. Doll & Marcus Conrad
Comprehensive Cancer Center Mainfranken, Universitätsklinikum Würzburg, Würzburg, Germany
Viktoria Kunz, Kurt Bommert & Ralf C. Bargou
Department of Biochemistry and Molecular Biology, Theodor Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany
Werner Schmitz
Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle, Germany
Aline Horling & Peter Imming
Department of Biological Sciences, University of Denver, Denver, CO, USA
Ann M. Wehman
Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
Thiago C. Genaro-Mattos & Karoly Mirnics
Faculty of Applied Sciences and Biotechnology, Shoolini University, Himachal Pradesh, India
Lokender Kumar
Department of Physics, Colorado School of Mines, Golden, CO, USA
Judith Klein-Seetharaman
School of Molecular Sciences, Arizona State University, Phoenix, AZ, USA
Judith Klein-Seetharaman
Department of Pathology, University of Würzburg, Würzburg, Germany
Svenja Meierjohann
Medizinische Klinik und Poliklinik IV, Ludwig Maximillian University, Munich, Germany
Isabel Weigand & Matthias Kroiss
Institute of Experimental Cancer Research, University Hospital Ulm, Ulm, Germany
Georg W. Bornkamm
Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
Fernando Gomes & Luis Eduardo Soares Netto
Department of Pharmacy, Ludwig Maximilian University of Munich, Munich, Germany
Manjima B. Sathian & David B. Konrad
Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA
Douglas F. Covey
Taylor Family Institute for Innovative Psychiatric Research, Washington University, St. Louis, MO, USA
Douglas F. Covey
Research Unit Analytical BioG

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## Expert Analysis

### Merits
- Doll & Marcus Conrad Comprehensive Cancer Center Mainfranken, Universitätsklinikum Würzburg, Würzburg, Germany Viktoria Kunz, Kurt Bommert & Ralf C.

### Areas for Consideration
- Following identification of the issue, we reanalysed the data to evaluate the potential impact of the blank values on our results, employing two distinct methodologies.

### Implications
- Following identification of the issue, we reanalysed the data to evaluate the potential impact of the blank values on our results, employing two distinct methodologies.
- These corrections do not affect the experiments and the study conclusions.
- Nature (2026). https://doi.org/10.1038/s41586-026-10403-z Download citation Published : 24 March 2026 Version of record : 24 March 2026 DOI : https://doi.org/10.1038/s41586-026-10403-z Share this article Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article.

### Expert Commentary
This article covers author, view, publications topics. Notable strengths include discussion of author. Areas of concern are also raised. Readability: Flesch-Kincaid grade 0.0. Word count: 1368.